I am going to perform protein determination on an oil palm biomass sample. Among the two methods, Lowry and Bradford, which one is more convenient? Can anyone share their experiences?
Hi,
I am not sure which method is more convenient. This will I am going to do to down select them.
1. Which one will give you more accurate value
2. Which method is used more frequent in the literature. This will make your data can compare with other's.
Lowry is not the method of choice if your sample contains fat...threfore I would prefer Bradford
Both the methods have advantage and disadvantage depending on the extract. For non-phenolics lowery is good.
Bradford is the most preferable method as lowry method has interference due to lipids, certain buffers, drugs, lipids, sugars, salts, nucleic acids and sulphydryl reagents
these 2 methods have advantage and disadvantage .I recommend to use kjeldahl method.
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