Protein BCA assay question: DDM/CHS interference and native/denatured protein measurement

01 January 1970 1 7K Report

Hello everyone,

I'm currently conducting the BCA assay for a membrane protein present in a solution containing 1XTBS buffer, 10% glycerol, and 0.2% DDM/CHS (in a 10:1 ratio). I have a couple of questions regarding this setup:

Does the detergent DDM/CHS interfere with the BCA assay in any way? I could not find them in the list of chemicals provided.

Does the BCA assay measure the native state of the protein, denatured protein (treated with SDS), or both? If it measures both, would the protein assay yield different results when the protein is in its native state compared to when it's denatured?

I would appreciate any insights or experiences you may have on these matters. Thank you in advance for your help!

Adam B Shapiro

Detergents should not interfere with the BCA assay. The conditions of the assay are moderately strong base, which should denature most proteins. So, in general, the denatured state is measured.

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