Pawan Bhatta

3 Questions 1 Answers 0 Followers

Questions related from Pawan Bhatta

What is in my protein in this agarose gel?

Hi everyone, My 260/280 ratios were higher and I suspect contamination of DNA or RNA. Is is DNA or RNA in the agarose gel? The shape looks moon shaped. The image looks like following:

07 November 2024 3,095 3 View

What does it mean by abolished binding (ki or kd experiment) of ligand but x-fold reduction ec50 or ic50? Should it not be no binding no ec50?

I was going through GPCR binding database. I was confused to see that a agonist can have abolished effect on E169A mutation for a ligand CGS21680 using kd determination experiment (paper PMID:...

03 September 2022 1,024 0 View

Protein BCA assay question: DDM/CHS interference and native/denatured protein measurement

Hello everyone, I'm currently conducting the BCA assay for a membrane protein present in a solution containing 1XTBS buffer, 10% glycerol, and 0.2% DDM/CHS (in a 10:1 ratio). I have a couple of...

01 January 1970 7,046 1 View