Pawan Bhatta

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Questions related from Pawan Bhatta

What does it mean by abolished binding (ki or kd experiment) of ligand but x-fold reduction ec50 or ic50? Should it not be no binding no ec50?

I was going through GPCR binding database. I was confused to see that a agonist can have abolished effect on E169A mutation for a ligand CGS21680 using kd determination experiment (paper PMID:...

03 September 2022 1,020 0 View

Protein BCA assay question: DDM/CHS interference and native/denatured protein measurement

Hello everyone, I'm currently conducting the BCA assay for a membrane protein present in a solution containing 1XTBS buffer, 10% glycerol, and 0.2% DDM/CHS (in a 10:1 ratio). I have a couple of...

01 January 1970 7,043 1 View