I have some problems with doing the calculations for protease enzyme activity after using this protocol.
Universal Protease Activity Assay using Casein as a Substrate (sigmaaldrich.com)
Is anyone familiar with this protocol and can offer some assistance on the calculations? Any assistance would be appreciated. If i have an idea of how it's done, I will be able to complete the rest of my calculations. The explanation for doing the calculations is a bit confusing.
Thank you in advance.
Also, to those who answered my last post thank you. I was able to grasp both Amylase and Lipase enzyme activity calculations.
are you using the assay that employs the use of 0.65% Casein? Do you establish a Standard Curve using 1 mmol/ml Tyrosine? If that is the case and you have run a set of standards from 0.05 mmol/rxn to 0.5 mmol/rxn, the calculations are relatively straight forward. See attached.
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