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Protein Inhibition Denaturation Assay: No absorbance detected at 660nm?

Methods are based on Chel-Guerrero and colleagues'Phytochemical Profile, Toxicity, and Pharmacological Potenti... . We use a 1M Tris buffer with pH of 6.8, with Bovine Serum Albumin as the...

25 April 2024 8,802 0 View

What agar substitute can I use to culture Thermophiles at 80 to 95 °C so as to view colonies?

I will be working with thermophiles, and I want to culture them on solid media, and view colonies. However, traditional agar melts at lower temperatures, and my desired temperature is 80 °C

16 November 2022 2,209 1 View

Cell lysis with Triton X-100 ?

-does Triton X-100 also permeabilize nuclear membranes? -what is the ideal concentration of Triton X-100 to use? -do low temperatures slow down or prevent cell lysis (by Triton)?

09 August 2022 1,899 0 View

What method can I employ in washing hydroxyapatite to make it neutral?

I synthesized hydroxyapatite from an agroindustrial waste using the wet precipitation method. However, after synthesis, my material is still basic after several wash with deionised water and...

26 January 2022 7,766 5 View

Is there any standard thickness for scaffolds used in bone tissue engineering?

Bone tissue engineering

04 July 2021 1,387 4 View

How to find the eigenvectors of a matrix function that is linear in its eigenvectors?

Be A(u) = ( u1 * A1 ) + ( u2 * A2 ) + ( u3 * A3 ), u = [ u1 u2 u3 ]T, Ai are known 3x3 real matrices, i=1,2,3. How to solve the following equation: Find the scalar \lambda and the vector u such...

08 March 2021 2,257 6 View

¿Me puede ayudar con una guia para realizar una simulacion en Ansys Fluent, un analisis de flama de combustion premezclada de un quemador de gas ?

La premezcla sera de aire con propano

30 May 2020 1,557 0 View

Is there any kind of interference between wireless networks?

during my day to day I could appreciate how the intensity of the wifi networks are altered because of the existence of other networks, here is my question because I want to know if. Is there any...

05 July 2019 5,167 5 View

Can anyone explain the activation/inactivation curves that are used to describe 'incomplete inactivation' and the 'window current' in LQT3?

I'm referring to the overlapping sigmoidal curves that are used to describe the gain-of-function mechanisms. I understand the activation curve, but I can't seem to get my head around the...

08 March 2019 1,584 3 View

Where can I find a model of anorogenic rapakivi intrusions?

I am modeling with gravimetry a small section of the rapakivi granite of Parguaza, Venezuela. I am located near from the left flanc of the Parguaza graben so I am also in an extensional setting....

09 September 2018 9,578 8 View

Why citations are not recorded on my profile researchgate web?

Dear colleges , I would appreciate if you have a time to help. I received mails told me there are numbers for research articles. These citations are not recorded on my profile. Regards, Sherif

09 July 2024 6,195 1 View

Why don't I have a PCR product with proofread polymerase?

Dear Colleagues, I have the following problem: I’m trying to amplify a cassette with a resistance gene (about 2 kb) from the yeast genome, so that I can then insert it into a plasmid. With Taq...

03 July 2024 105 11 View

In what ways does the selective integration of emerging technologies enhance customer satisfaction on direct-to-consumer (D2C) platforms?

In the rapidly evolving landscape of e-commerce, D2C platforms are increasingly leveraging emerging technologies to streamline their operations and provide exceptional customer experiences. The...

04 June 2024 5,231 0 View

Link of Reserach gate with signatures ?

How to add the Link of Research Gate with Signature, while I mail to all.

27 May 2024 886 1 View

Any postdoctoral fellowship or Research Associate opportunity ?

I need a Postdoc fellowship or Research Associate opportunity in Language Education and related areas. I am a vibrant researcher with these areas of interest, among others; English language...

23 May 2024 2,720 2 View

How to create account on research gate by personal mail id ?

Without using institutional mail id

10 May 2024 9,203 3 View

How to fix wrong authors of a publication in ResarchGate system ?

There is a serious problem about the authors of a recent publication of my reasearch team. Wrong authors have been automatically registred in ResearchGate : Angelo Castrorao Barba, Pilar...

03 May 2024 992 2 View

What your status?

Dear Jose, Hi, are you working or retired. I still work as the professor at BSUIR. My mail [email protected]. Prof. Vishniakou, Minsk.

27 April 2024 3,461 1 View

What do to change profil?

What o do when I lost ma password and want to change my mail adress as beein retired?

24 April 2024 8,633 1 View

Could you please recommend sources for an internship pre-service teachers reflection paper?

Greetings community. Are there any experts in this topic you can recommend to build a solid theoretical revision for a reflection around the topic? Thanks for your help.

10 April 2024 1,592 2 View

Maura Zimmerschied

Native speakers of the written language are likely to do better. If you can find one with some background in the subject area, their work may identify ambiguities and provide more suggestions for improvement.

Marcelo André Machado

I have had a good experience with American journal experts - AJE. I will try SAGE MILES next time.

Jeon Hwan

proofstep is a really good option. I have used them multiple times with good results. www.proofstep.co.uk