Dear All,
How often did it happen that you have one anticancer drug which is evidently affecting the clonogenicity of cancer cells whereas when you check its effect on the same cancer cells growing in "liquid culture", they have really subtle/minor effect if any?
How do you explain this?
Tomas Koltai
The microenvironment has a capital importance in how a cytotoxic compound works.
Cell culture in plates have different environment than liquid cultures. And both are very different from in vivo conditions. A slight change of 0.1 in pH may change the effect.
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Khumukcham Saratchandra
Dear Amal Kamal Abdel-Aziz ,
How did you perform clonogenic assay and how are you going to distinguished from "liquid culture". Is liquid culture means suspension growth or normal growth?
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