24 December 2021 4 4K Report

profile distribution

More Okoye Ruby's questions See All
I am new to cell culture technique and reading papers on cell culture.Is there any criteria for selecting certain cell culture (cell line)?

I need to select a cell type to start my experiment. My experiment is on female genital tract infection and I intend to see cytokine expression. I have read about J774 but I do not know why to use...

09 August 2020 8,504 3 View

Need help- how to use primary and secondary data in same paper in regression?

Actually am trying to use regression for my paper where am drawing primary data questionnaire(independant)from few universities students and trying to establish relations with student...

02 May 2020 7,097 3 View

Can anyone help me convert the concentration of human anti-hepatitis B antibody (Hepabig) from IU/ml to mg/ml?

I am using Hepabig brand which only states 100 IU/0.5 ml concentration of antibody but no mg/ml immunoglobulin concentration estimate.

05 March 2019 5,808 15 View

How to tell apart primordial germ cells from EG cells?

Is there any method that can tell apart EG cells from primordial germ cells? By morphology or surface marker, gene expression, et cetera. ?

11 November 2018 2,117 3 View

How to test purity of cultured macrophages with flow cytometry?

What's a good strategy to gate macrophages cultured from isolated bone marrow cells? Available antibodies are CD115, CD11b, F4/80 and Gr-1.

31 October 2018 1,529 2 View

How do I deal with recombinant protein seperation after MBP Factor Xa cleavage digestion of recombinant fusion protein?

After purifying my recombination fusion protein sample using a Bio-rad Q-column chromatography, the resultant samples, were verified via SDS-PAGE analysis and stored in glycerol 20% concentration...

29 July 2018 5,888 3 View

Is there a simple method to use pslegend (GMT) to create a legend for 2-D plot?

I am new for Generic Mapping Tool (GMT), but I would like to work through a few simple examples of generating legends (ie. multiple points or lines plotted on a single figure using psxy). In the...

26 April 2018 8,197 3 View

What protocol could be implemented in analyzing for Total hydrocarbon content of soil using UV-Vis Spectrophotometer?

I need to determine the THC of crude oil polluted soil.

15 March 2018 7,557 6 View

Hi, is it possible to observe increase in cell viability of normal fibroblast cells (NIH3T3) with increasing biosurfactant-coated iron oxide NPs?

I have repeated the experiment twice (in triplicates/experiment) and have observed increase in viability with increased nanoparticle concentration (100 ug/ml-1 mg/ml). Can anyone please help me...

15 March 2018 8,589 2 View

How to use high precise geoid for mineral exploration?

Recently, many scientific applications such as: In geology, the inversion of the geoid is used for Petroleum Exploration. I am looking for studies and research in this field

01 January 2018 2,539 4 View

Similar questions and discussions
Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

I want to simulate a system containing protein and heavy metals; which force field is most reliable for this purpose?

The protein I want to simulate has multiple heavy metals. I wanted to know which force-field I should use with GROMACS to achieve this. Most importantly, do the latest updates in the force-field...

16 February 2021 2,420 1 View

Aspen Plus Error: Unknown or undefined bead number?

Hello, in my Aspen Plus Simulation (see attachment) there are two heat exchangers. One HEX1 (oil+gas) ist fed by hot gas which ist coming out of a burner. The other HEX2 (gas+gas) shall work as a...

14 February 2021 8,096 2 View

What can be use to mop up heavy metals from vegetables or soils in quarry mining site?

It has been established that quarry mining site polluted and contaminated it's environment with heavy metals, these affects the crops and vegetables planted in the area. The possible solutions to...

12 February 2021 1,155 5 View

Can downdraft gasifier for RDF be scaled up?

Dear researchers, In literature, so far I am seeing that downdraft is usually used for small-scale purposes (10kW-10MW). One of the reasons behind is "they do not allow for uniform distribution...

08 February 2021 5,403 1 View

Please how can i write a proposal on the methodolgies of AAS to determine heavy metalsin water?

Please I want to write a proposal on the methodologies of AAS to determine heavy metals in water. please any link can help.

08 February 2021 3,587 7 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close