I am wondering if anyone can help me.  I have some cadaver skin tissues that were processed, however I did not get proper infiltration.  I used an extended human process (etoh 70 - 2 hr, etoh 80 - 2 hr, etoh 95 - 2 hr, etoh 100 - 4 hr, xylene A - 1 hr, xylene B - 1 hr, xylene C - 2 hrs, wax A - 1 hr, wax B - 1 hr, wax C - 2 hrs).  I am wondering if there are anything protocols for processing that will help with infiltration.  Or if anyone has any tips to share for dealing with difficult cadaver specimens (specifically skin).

Thank you all!

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