Data signal through a twisted pair cable by using different color codes will work ?

will a Data signal through a twisted pair cable by using different color codes will work ?. I am using a shielded twisted pair cable for my Mod bus device. Instead of same color pairs for Data...

02 May 2019 6,361 3 View

Can anyone suggest a good method/paper to mass produce TEV protease ?

I am planning to produce TEV protease in my lab because I will need a lot of the protein in order to cleave the fusion tag off the recombinant proteins which I will be producing in E. coli. Do...

03 November 2016 6,281 3 View

Is the numerical value of positron diffusion constant different from surface to bulk of the solids?

If I consider positron diffusion length L+ is constant through the solid. But by VEPFIT I am getting two layer (surface and bulk) of the solid

02 February 2016 1,270 1 View

Is there a colorimetric assay to quantify terpenoids from plant extract? Which is the best way to quantify terpenoids apart from GC-MS analysis?

Apart from GC-MS analysis which method would be best to quantify terpenoids from plant extract?

27 March 2015 7,764 8 View

How can one obtain a better yield from Palm oil fractionation? Is 85% achievable on a PO IV of 53?

What would the cycle time be?

02 December 2014 8,369 3 View

Mass Spec Vs 3D structure role in deamidation identification. Which method is more accurate ?

24 September 2014 9,154 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

How can I measure the surface interaction or surface interfacial chemistry between polishing abrasives and upper surface of substrate ?

I am working on polishing abrasives. I need to do few characterizations ? which can provide the on time surface chemistry of substrate with polishing abrasive during optical polishing. I would...

18 February 2021 5,319 3 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View