What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

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Please recommend the optimal optical component and its parameters. Thanks a lot

21 December 2020 7,678 3 View

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02 December 2020 7,293 2 View

The titanium electrode corrodes during the ohmic heating process, will it affect the electrical conductivity of the sample?

The titanium electrode corrodes during the ohmic heating process, will it affect the electrical conductivity of the sample? Thank you .

19 November 2020 4,082 2 View

How to measure the focusing efficiency of Fresnel zone plate (FWHM is in micrometer scale) ?

We want to measure the focusing efficiency of the Fresnel zone plate with a very small FWHM. "The focusing efficiency is defined as the ratio of the total power in a circular aperture with a...

01 November 2020 1,252 7 View

How to use first-order markov model predicting continuous time-series data?

Hello, everyone, I want to use markov model to predict continuous time-series data, and I read some references, It seems that the model is more suitable for discrete data , so I divided the...

06 October 2020 8,239 3 View

How can we do measurement using SEM?

SEM samples are different in height, ranging from several millimetres to tens of millimetres, and the vertical position of the SEM stub can be changed if we wish to. How do we determine the actual...

05 September 2020 7,152 5 View

How to do SEM for fiber reinforced cementitious material?

After a bending test or direct tensile test, a specimen fails and forms a crack. I would like to investigate the morphology of the cracked surface using SEM. How do I prepare the sample correctly?...

02 September 2020 9,539 13 View

How can I accelareate dehydration of mouse lung with 10% sucrose for frozen section?

09 August 2020 4,967 1 View

How to calculate monthly max and min temperature of ERA-40 dataset in Matlab?

Hello, everyone. I have downloaded the daily 2m temperature of ERA-40 dataset, it's .grib format. I want to calculate monthly max and min temperature, but now the data record four times a day ,...

27 May 2020 7,000 5 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View