Problems with MIC testing of VRE in MHB – alternative growth media?

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

How to calculate FIC index?

Can someone explain to me how was the MIC of MO in combination and MIC of antibiotic in combination was calculated? as shown in the attached file below.

26 July 2024 2,702 1 View

Is there any Impact of Interest Rate, Corruption Perception Index, and Economic Growth on Foreign Direct Investment in Developing Countries?

Is there any impact of the interest rate, corruption perception index, and economic growth on foreign direct investment in developing countries?

07 July 2024 770 7 View

Why do we use Horse Serum for C2C12 cell differentitation?

I have seen some papers that differentiate C2C12 myoblasts into myotubes by changing the growth medium that contains 10%FBS by a medium containing 2%Horse serum/calf serum + 1% NEAA. What's the...

04 July 2024 4,732 1 View

How do I make a solution from fibroblast growth factor since it is available as a powder?

I am using fibroblast growth factor, purchased in powder form. What suitable substance should be added to get it in a liquid form? Thanks

03 July 2024 9,866 2 View

Root Phenotypes in Arabidopsis Col-0 Under Phosphate Deficiency Conditions not showing up??

Introduction: I am conducting an experiment to investigate the root phenotypes of Arabidopsis Col-0 plants grown under phosphate deficiency conditions. Here, I describe my current methodology and...

19 June 2024 8,774 3 View

Why don't my intestinal organoids from children's polyps grow?

Hi! I am having trouble growing intestinal organoids from children's polyps. Sometimes they don't grow at all, and other times they look dark and unhealthy, as shown in the attached pictures...

18 June 2024 4,781 3 View

Question about ELISA with the "Cattle IGF 1 (Insulin like growth factor) ELISA Kit" Cat: ELK5630?

Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...

13 June 2024 9,473 0 View

Phil Geis

On what medium - what conditions - do you obtain adequate growth?

Shashank Kashyap

Dear Onnipa Hasangsri, You can try the BHI medium for the growth of VRE. And with respect to growth on MHB medium for MIC, you can try inoculating the bacterial colonies from the BHI plate and incubating at 37°C for 16-18 hrs.