I am working on DNA crystallography. Recently l have collected one data from Synchrotron, Grenoble, France. We are unable to process the data. It was noted that the phi angles of the images are not changed when l am loading to the iMosflm and XDS. Why it is so? Can you please suggest some possible ways to proceed with the data?
Dear Dileep,
DNA crystallography is in principal not different to regular protein or macromolecular crystallography. Your problem of not indexing or processing data can have many reasons. Without looking at the data I can not provide advise. Feel free to contact me.
Hello Dileep,
As Raimund said, it could be a number of things going wrong. As for the phi angles not changing, you check the headers of the images themselves to see a) what angle they think they were collected at, and b) what delta-phi is used. If the crystal did indeed rotate, you can manually input the delta-phi into the XDS.INP file or enter it into the experimental setup of MOSFLM. I'd also be willing to take a look at the data if you'd like.
- Badges
- Science topic
- Similar topics
- Biological Science
- Bioscience
- Biotechnology