No title

02 August 2020 7,331 3 View

I have created a survey for my research about leadership and change. https://www.surveymonkey.com/r/CB72MGCan you please help me by taking the survey?

https://www.surveymonkey.com/r/CB72MG6

08 February 2020 4,048 1 View

How to measure the quality matrices of a journal?

Many researchers and scientists are in confusion about judging the quality of a journal. Mere impact factor cannot measure all performance indicators of a journal. The following 5 indicators...

29 December 2017 9,392 9 View

Any one have or know about the availability of destabilized Green Fluorescent protein expressing cell line ??

Hi I m looking for destabilized GFP (deGFP) expressing cell line or vectors (viral vectors to make stable expressing cell line). I need this cell line to study the release kinetics of...

30 October 2017 225 3 View

RNA with low 260/230 =<1, showing two peaks, one close to 230 and 270?

Datasheet (Nanodrop 2000) and others said possibly these peaks are due to phenol contamination. Methods like 1- Butonol/ sodium acetate re-precipitations have shown to improved the purity of RNA....

25 July 2016 8,903 14 View

Can any one help me in analyzing the quarter car model developed using simscape toolbox in MATLAB?

03 February 2016 4,798 1 View

How can one prepare Ag Nanoparticles embedded glasses?

We have tried by heat treatment method and it requires some corrections. In which, batch chemicals caintaining silver nitrate were melted at 1100 degree and then poured on to te brass plate and...

07 June 2015 8,814 4 View

How can I find nanoparticle diameter in TEM image?

Is there any software available for that?

07 June 2015 5,427 2 View

How does one interpret high growth inhibition of an extract on a normal cell line?

16 March 2015 9,917 3 View

Which software can give me accurate 3D images from 2d photographs of an item and can also measure the dimensions from the 3D image ?

02 March 2015 4,695 15 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

How can I label an antibody to determine how far it is travelling from the microdialysis probe?

I am trying to set up a new experiment where I am simultaneously utilizing reverse dialysis (antibody) through a microdialysis probe and a Miniscope for one-photon calcium imaging. Both pieces of...

09 February 2021 3,981 1 View