I have attached the screen shot. In the table below It compares sequences between COVID-19, SARS-COV and Bat COV
Mismatch numbers 11 and 9, meaning that COVID-19 compared to the other two sequences has that many number of point mutations in the selected regions? and assays primers & probe are able to distinguish specifically those point mutations and fail to amplify incorrect template? if so why does the number decrease in other assay? those assays primers & probe give false positive?
Am I interfering it rightly.
https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045
Xin Pin hi assays primers & probe is very sensitive and the errors are due to several reasons: 1. Lack of dedicated connection primers & probe ( no setup )2- Contamination in the reaction and.....
Xin Pin If these point mutations (11 or 9 bases) scatter around in the genome (ex. SARS-CoV is 29,727 bp [1]), instead of clustering in small regions, it is very hard to design primer pairs for PCR to distinguish them ( COVID-19, SARS-COV and Bat COV ).
[1] https://www.cdc.gov/sars/lab/sequence.html
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