Hello,
In my research I use primary melanocytes, I often have them de-differentiating into melanoblasts in certain cultures, any suggestions of to avoid this? or what could be the cause of this event?
Any reflections would be appreciated!
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.
03 March 2021 5,266 4 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...
02 March 2021 9,833 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...
01 March 2021 8,220 2 View
I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....
01 March 2021 3,325 2 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?
28 February 2021 3,864 3 View
Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...
28 February 2021 2,116 1 View
Looking to use SNAP for some melanocyte cell work. The protocol sheet says to use deoxygenated buffers to make stocks but it also says it is soluble in PBS. So why do you need to go through the...
05 June 2019 6,616 2 View
Since MSM is an organo-sulfur compound, would it be reasonable to expect that it would influence skin pigmentation to some degree ?
11 December 2018 430 0 View
I am looking to counter stain mouse melanoma tumors with Azure B instead of hematoxylin since Azure B will stain melanocytes blue-green to allow differentiation from DAB during IHC. I am having...
08 September 2018 212 0 View
A lot of protocols are adapted to skin slices but nothing for 2D cell cultures. Thanks for your help.
05 June 2018 8,181 0 View
Also is it beneficial to propose a thesis and research this antagonist? An already discovered antagonist is met- Pro-D-Phe-Arg-D-Trp-Phe-Lys-Pro-Val-NH2 and it has been proven to reduce alpha-MSH...
03 April 2018 4,384 1 View
Most research papers I found only studied its effects on depression and Parkinson's disease rather than its ability to treat hyperpigmentation of the skin. I am beginning to this that it may be...
26 March 2018 5,378 2 View
02 March 2018 4,647 3 View
DOPA staining or Masson Fontana staining were positive for the melanocytes in the hair follicle tissue. I'd like to know whether IHC staining could be co-expressed with DOPA or Masson Fontana...
01 February 2018 2,744 0 View
I am interested in changes in my target gene expression during melanoma progression. So, I have used total RNA from melanocytes and 4 melanoma cell lines. I have taken 250 ng cDNA for single qPCR...
31 December 2017 3,770 4 View
And also how can we differentiated them (Ab, gene Expression...)
11 December 2017 7,073 0 View