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Questions related from Zahraa Fakih
I need to know if the promoter of a target gene of interest has a GAS sequence, any idea how the steps go? thanks in advance
24 May 2023 9,335 1 View
I'm trying to perform immunofluorescence staining of Dynein and kinesin on human skin, but I don't know how it should appear, and I looked through published apers and I didn't find any. Any help?...
06 May 2023 9,143 0 View
In my IF experiment I need a positive and a negative control of my target protein, so i need to know which tissues express the highest levels of the protein of interest and also the one with the...
03 May 2023 9,303 3 View
And how does the pt link retrieve the antigen? cz I searched that but I didn't find how exactly.
04 April 2023 2,151 2 View
I have diluted the primary and secondary antibody in SVF (5%) and i got a high background, then I tried with HS (2.5%) and still didnt work
02 April 2023 800 2 View
I usually fix cells with Formaldehyde 3.6-4% at 4 degrees, I was having a poor staining, may this be due to the crosslinking induced by formalin? additionally, what is the consequence if reducing...
01 January 1970 6,052 2 View
Hello, In my research I use primary melanocytes, I often have them de-differentiating into melanoblasts in certain cultures, any suggestions of to avoid this? or what could be the cause of this...
01 January 1970 4,156 0 View
Hello everyone, I'm currently working on immunofluorescence staining experiments, and I've noticed a consistent issue. Regardless of the primary antibody I'm using, I always observe higher...
01 January 1970 3,148 1 View