Zahraa Fakih

8 Questions 1 Answers 0 Followers

Questions related from Zahraa Fakih

Any idea how to find if the promoter sequence of a gene has a GAS sequence?

I need to know if the promoter of a target gene of interest has a GAS sequence, any idea how the steps go? thanks in advance

24 May 2023 9,343 1 View

Anybody have a photo for the immunofluorescence staining of dynein and that of kinesin in human skin epidermis?

I'm trying to perform immunofluorescence staining of Dynein and kinesin on human skin, but I don't know how it should appear, and I looked through published apers and I didn't find any. Any help?...

06 May 2023 9,161 0 View

Any suggestion of a site like a protein atlas to figure out the tissues in mice with the highest and lowest expression of specific proteins?

In my IF experiment I need a positive and a negative control of my target protein, so i need to know which tissues express the highest levels of the protein of interest and also the one with the...

03 May 2023 9,314 3 View

Is the purpose of the PT-link in Immunohistochemistry perform antigen retrieval only or also permeabilization of membranes?

And how does the pt link retrieve the antigen? cz I searched that but I didn't find how exactly.

04 April 2023 2,160 2 View

Regarding immunofluorescence on mice skin tissue, I tried using HS (2.5%) & SVF (5%) but for both i keep on having a strong background, sugestions?

I have diluted the primary and secondary antibody in SVF (5%) and i got a high background, then I tried with HS (2.5%) and still didnt work

02 April 2023 810 2 View

Immunocytochemistry staining of loosely attaching cells

I usually fix cells with Formaldehyde 3.6-4% at 4 degrees, I was having a poor staining, may this be due to the crosslinking induced by formalin? additionally, what is the consequence if reducing...

01 January 1970 6,063 2 View

Primary melanocyte culture issues

Hello, In my research I use primary melanocytes, I often have them de-differentiating into melanoblasts in certain cultures, any suggestions of to avoid this? or what could be the cause of this...

01 January 1970 4,163 0 View

High Background Staining in Epidermis vs. Dermis During Immunofluorescence

Hello everyone, I'm currently working on immunofluorescence staining experiments, and I've noticed a consistent issue. Regardless of the primary antibody I'm using, I always observe higher...

01 January 1970 3,155 1 View