Hello,
I would like to prepare hyperglycaemic DMEM culture medium with 30mM D-glucose. How do I go about that please? As in, if I want a final volume of 600 ml of culture medium,how much glucose would I include please?
Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...
16 February 2021 453 2 View
Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...
07 February 2021 9,989 7 View
Hello, I'm now working on C2C12 myoblast cell line. Growing the cells was great, but there are some problems during differentiation process. The cells are not differentiating but frequently...
30 January 2021 9,535 2 View
Dear all, I am trying to simulate the enzymatic hydrolysis of sucrose with invertase in CSTR in Comsol. The reaction pathway is such that Sucrose + Water -> Fructose + Glucose. It is desired...
27 January 2021 2,525 4 View
Hello everyone. I am curently working on a protocol to measure glucose uptake in HepG2 cells using 2-NDBG. The work involves the pre-treatment of cells with an active material and observe if it...
25 January 2021 7,608 3 View
Three days ago, i thawed Hela cancer cells from liquid nitrogen but until now they are still suspended in the media and very very low number of cells became attached to the tissue culture...
06 January 2021 6,511 4 View
I read a binding buffer in references. The recipe was listed as follows: Glucose (4.5g/L), Magnesium Chloride (5 mM), yeast tRNA (0.1 mg/ml) and BSA (1 mg/ml) was dissolved in DPBS. This buffer...
23 December 2020 8,440 4 View
I have measured levels of GLUT1, GLUT3, and GLUT4 in the mouse hippocampus and cortex using ELISA kits. I'm trying to find typical concentrations (in ng/mg total protein) of these proteins in the...
21 December 2020 4,647 2 View
Hi, I've determined the glucose content in Cellic CTec2 enzyme blend and it is 12-14 mg per 100 mg of enzyme blend. Could anyone share with me your results so I can compare it with mine?
08 December 2020 6,312 3 View
I have been working with the C2C12 myotubes and measured their glucose uptake activity using an indirect glucose uptake assay (measuring how much glucose is left in the medium after stimulating...
23 November 2020 2,118 3 View