Hi guys
Does anyone have the experience to perform preparative TLC with RP-18 as a stationary phase? What kind should avoid in order to not dilute the stationary phase when I extracted my target compound after separation?
Check https://www.mn-net.com/tlc-glass-plates-rp-18-w-uv254-c18-modified-silica-layer-20x20-cm-preparative-811074
https://www.merckmillipore.com/RU/ru/products/analytics-sample-prep/chromatography-for-analysis/thin-layer-chromatography/tlc-plates-thin-layer-chromatography/rp-modified-silica-plates/rdmb.qB.ITwAAAFAjvtkiQpx,nav
Also check https://www.sciencedirect.com/topics/chemical-engineering/thin-layer-chromatography
https://pubmed.ncbi.nlm.nih.gov/33673150/
thanks for your respond
How to make stock solution which diluted into various concentration (ex. 10, 20, 30 ppm) and added by DPPH solution. Then read by spectrophotometer UV-Vis? Should I sentrifuge the solution of...
10 December 2019 8,354 5 View
Verification on pharmacopoeia method on related substance. Is it necessary to verify the related substance method on 'accuracy' using spiking method?
06 December 2016 5,236 3 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...
02 March 2021 2,735 2 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.
01 March 2021 4,306 1 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...
01 March 2021 8,079 2 View
I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...
28 February 2021 6,477 1 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View
Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.
28 February 2021 3,640 3 View