I'm using HepG2 to form 3D HepG2 aggregates with alginate, to confocal microscopy analyzes. However, I have difficulty to maintaining the spheroids after fixation and all procedures of incubations with antibodies. Has anyone had this difficulty? Could you help us?
Saleh Alkarim
Hello
kindly look at this paper ( Fabrication of three-dimensional porous cell-laden hydrogel for tissue engineering ) . which it suggests that porous alginate hydrogels resulted in formation of larger spheroids and higher albumin secretion compared to nonporous conditions and may have provided a better environment for cell proliferation and albumin production.
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