I have to load a aspirin in the polycaprolactone (PCL). Both, polymer and aspirin will be dissolved in the dichloromethane(DCM) to prepare the drug loaded mixture. After that, the mixture will be used to prepare the multiple parts in the cube shape. I want to know the amount of the drug loaded into the each part. Is UV spectroscopy the right solution?
If I have to go through the UV spectroscopy, then the how can I make a calibration curve? Should I prepare the calibration curve by dissolving the known amount of drug in the DCM or by dissolving the known amount of drug in PCL+DCM solution.
Please clear me out this doubt.
Dear Sir,
Good day. Yes you can check your aspirin using UV method, provided that you are not facing any matrix interference from your sample. According to USP you can detect aspirin using wavelength 280nm.
In order to eliminate the matrix effect, it is better that you include PCL into your calibration solution. You can prepare you calibration solution by adding known amount of aspirin into a fixed amount of PCL and DCM solution, and always perform a blank check using PCL and DCM.
By doing that any value contribute from PCL and DCM can be subtracted, giving you absorbance value of aspirin only.
If the interference can't be eliminated by matching the matrix, you have to change to HPLC method for a better analysis or search for a better wavelength that gives better result.
Hope this helps your study.
I agree with Ooi Yan Jie answer but I would also measure different dilutions of PCL in DCM, and fixed aspirin concentration in DCM with variable PCL to check for possible interference.
I have read in previous research that PCL does not have any wavelength spectrum in the range in which aspirin is being detected
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