Do you recommend an effective anti-OCT4 Ab for IHC?

I'm staining some brains with several stemness markers but the OCT4 antibody that I have been using is not working. I would really appreciate your recommendations! Thank you!

25 January 2021 3,169 3 View

Why does bromophenol blue band seem to split up during this SDS PAGE run?

06 January 2021 7,788 4 View

Why do I keep obtaining the same secondary PCR band along with the specific product band?

Hello everyone! I need to amplify a specific DNA fragment in order to do Sanger sequencing for the detection of a point mutation in human patients. The fragment is in an exon of my gene of...

25 December 2020 1,805 3 View

How is possible that only a small percentage of bromophenol blue started the run in agarose electrophoresis ?

I used an 1,5% agarose gel for a second time and I loaded in the wells that I never used 15 microlitres of sample (constituted of 3 microlitres of bromophenol blue 6X, 6 microlitres of sample and...

07 December 2020 1,373 3 View

Is it correct to expect the same quantitative result between SDS-PAGE and BN-PAGE analysis performed on the same sample?

Please, can anyone please help me with this issue? By BN-PAGE and western blot on DDM-solubilized mitochondria, isolated from cultured cells, I found that in treated cells the amount of CV...

05 December 2020 7,696 2 View

How can I calculate degrees of freedom and write F for repeated measure ANOVA?

How can I calculate and report degrees of freedom for repeated mesure ANOVA? I have 48 observations N=48 and 2 factors of 3(P) and 8(LA) levels. I calculate degrees of freedom as follows: dF P =...

19 November 2020 5,682 5 View

What is the best pain killer for mice?

Good morning, I need to implant chronic electrodes in mice muscles. What is the best injectable painkiller to use during and after the surgery? and what the recommended dose for mice? And for how...

01 November 2020 8,477 1 View

Can the ramp rate of a Thermal Cycler machine influence the PCR result?

Hello, I am running PCR for genotyping. I didn't have problem till this Summer, when I couldn't see any band on the gel. I checked primers, Taq, etc. I bought new stocks etc. But still I can't...

16 September 2020 7,660 5 View

How to clean platinum/iridium single electrodes for re-use?

Hi, I will buy platinum/iridium electrodes, for single unit recording and stimulation. Since I am going to re-use them in several sessions: 1) How can I clean them? 2) Should I buy some equipment...

03 April 2018 5,828 6 View

Single electrode MICROMANIPULATOR. Which is the best?

25 March 2018 4,179 2 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

01 March 2021 3,905 5 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View