Paola Salmas

6 Questions 9 Answers 0 Followers

Questions related from Paola Salmas

What is the best pain killer for mice?

Good morning, I need to implant chronic electrodes in mice muscles. What is the best injectable painkiller to use during and after the surgery? and what the recommended dose for mice? And for how...

02 November 2020 8,626 1 View

Can the ramp rate of a Thermal Cycler machine influence the PCR result?

Hello, I am running PCR for genotyping. I didn't have problem till this Summer, when I couldn't see any band on the gel. I checked primers, Taq, etc. I bought new stocks etc. But still I can't...

17 September 2020 7,899 5 View

Prenatal preparation on RATS: embryo explant method. What is the best anesthesia?

Hello, I need to collect some E18 embryos from rat, for some control experiment on motor developing. I have never worked on embryos before so I am new to the technique. Can someone please...

05 December 2019 3,295 0 View

Ketamine/xylazine cocktail dose for Thy1-Chr2-YFP mice?

I am using a cocktail of 1ml ketamine, 0.5 ml Xylazine and 8.5 of saline, to anesthetize Thy1 transgenic mice. Lately the anesthesia doesn't seem to work properly, and I spend a lot of time to...

30 May 2019 9,076 3 View

How to clean platinum/iridium single electrodes for re-use?

Hi, I will buy platinum/iridium electrodes, for single unit recording and stimulation. Since I am going to re-use them in several sessions: 1) How can I clean them? 2) Should I buy some equipment...

04 April 2018 5,960 6 View

Single electrode MICROMANIPULATOR. Which is the best?

Hello, I am going to ourchase a micromanipulator for single elctrodes recordings. Which do you suggest me among these 3: - "EPS™ - Electrode Positioning System", ASlphaomega - Motorized...

26 March 2018 4,303 2 View