Oh thank you.. But i am working on endophytic fungi. I have isolated 20 endophytic fungi out of this i would like to choose 1 fungi which is rich in medicinally valuable metabolite to continue my further research. So for this suggest me some screening method.
You might as well screen all isolates against different microorganisms. In this way, you will have an excellent basis on what to select and study further (probably the one with broadest activity). You can simply test the crude extracts or perform dual cultures.
you can either put multiple different crude extracts in agar wells having a bacterial lawn OR streak different test organisms around the growth of a fungal endophyte. in both cases, you are looking for clearing zones.