Dear Sir. Concerning your issue about the prediction of peptide function. Various peptides are recommended for burning fat and building muscle. First, what are peptides? They are defined as a compound of two or more amino acids in which a carboxyl group of one is united with an amino group of another. With the elimination of a molecule of water, a peptide bond is formed. To put it simply, they are basically just small proteins. Technically, anything with fewer than fifty amino acids is considered a peptide although this is not hard and fast rule. A dipeptide is made up of two amino acids joined by one peptide bond. Tripeptides are three amino acids bound by two peptide binds. And so forth and so on. A polypeptide is just a long, un-branched chain of amino acids joined by peptide bonds that is not complex enough to be referred to as a protein. (Proteins are made up of polypeptides.). So, intake and production of all the amino acids is necessary for production of all the peptides needed for the body to work efficiently. Of course, as we age and we go through different times in our lives with different diets, stress, and physical changes some essential amino acids and peptide production is down-regulated. For example, insulin-like growth factor (IGF-1) production is reduced naturally when we are stressed and as we age. Peptides have many functions in the body, some act like neurotransmitters, others like hormones. Many control and influence how our bodies react to diet and physical exercise. There are also several amino acids that are necessary in sufficient amount to produce hormones like human growth hormone (HGH, pictured to the right). If you aren’t absorbing or making enough of those amino acids, your production of that hormone will be lowered. Those of us interested in fitness and athletic goals, of course, are interested in counteracting these down-regulations so that we can maximize our bodies ability to recover, perform, and meet athletic goals. I think the following below links may help you in your analysis:
So, I think it was not clear my question. I've so much peptide sequences, like: LEEGEWE From a digestion of a complex protein mixture. Well, I would like to predict the function of this peptide.
Some colleagues showed me the: http://www.uwm.edu.pl/biochemia/index.php/pl/biopep
The prediction of the function of previously unknown peptides is very difficult. The easiest way is to screen the literature, whether the respective peptide was already examined in a study. In addition, you have to define, what you want to know: natural function in cells, function as a food additive, function as a pharmaceutical compound, function as an enzyme inhibitor and not to forget the biological species. You also have to consider that many function assignments in databases or somewhere else are often very preliminary and may not be validated.
I did a hydrolyzate of a plant seed with the alcalase enzyme and a antioxidant activity test, it was positive. So I did mass spectrometry with this hydrolyzate and got those peptides.
So I would like to know which of these peptides is probably the holder antioxidade activity.
Unfortunately, I think that none of your peptides shows this activity. There are many other compounds, which may have antioxidant activity in seeds, e.g. tocopherols. To examine such mixtures, bioassay-guided fractionation (see review) might be the best way.