I am trying to establish an LC-MS/MS assay of my drug in SD rat organ homogenate. 5% TCA was used for protein precipitation. The assay was linear with good LLOQ for lungs and kidneys. However for liver and heart, I see some greenish gel like precipitate appearing. I tried acetonitrile alone and with methanol in several percentage, but the recovery of my drug is poor. Can someone help me?

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