Hello,

I have pre-sectioned slides of snap frozen tissues that were stored in OCT. They are currently at -80 degrees. The slides were not fixed prior to being stored in the -80. It was simply snap freeze the tissue, place in OCT, cryo-sectioned, store in the -80. I want to perform H&E and IHC (on two different slides of course).

Question 1: For H&E, do I air dry the slides first and then fix the slides (95% EtOH)? Or do I fix the slides when they are cold directly from the freezer? Does the fixative need to be at room temperature (95% EtOH), or should it be cold?

Question 2: For IHC, do I air dry the slides first and then fix the slides (cold acetone)? Or do I fix the slides when they are cold directly from the freezer?