Potassium-sensitive electrode interference

18 February 2016 1 7K Report

Hi,

Does anyone have experience with measuring extracellular potassium using valinomycin-based K+-sensitive micro-electrodes in conjunction with noradrenergic receptor antagonist application (Prazosin and proporanolol) in brain slices or in vivo? I know that these electrodes are sensitive to mono-amines, in addition to K+, but do anyone have experience with specific receptor antagonists?

Thanks for your time,

/Rune

Rogier Min

Hi Rune,

No, no experience with that. However, it would be quite straightforward to add a step to your electrode calibration procedure where you add the antagonist at the dose you want to use to your calibration solution. That would show whether there is interference at the antagonist concentration you want to use.

Best,

Rogier

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