Has anyone used LR White Resin for post-embedding IHC? Do you dehydrate the sample until 90% ethanol before inclusion?
I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....
29 July 2024 5,961 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
Many have criticized Noam Chomsky’s theory of language (e.g., Pinker as described in Sihombing 2022), but the most effective criticisms have come from Daniel Everett, given that Chomsky (according...
15 July 2024 492 4 View
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
14 July 2024 177 4 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
I have mouse lung tissue which is mounted with egg whites.I need to stain my tissues with PAS and HE. Can egg whites interfere with these stainings?
02 July 2024 8,261 0 View
In the context of doctoral research, I utilized immunohistochemical (IHC) analysis to quantify the expression levels of the Ki-67 proliferation marker. While the application of this test and...
25 June 2024 7,825 2 View