Hi everyone,
In our work with Bradyrhizobium spp., we normally include yeast extract to supply growth factors. However, in epifluorescence examination of cultures, the yeast extract causes high background levels of fluorescence (perhaps due to DNA remnants?).
I'm wondering if anyone has experience in substituting commercially available mixtures of vitamins (or other preparations) for YE in microbiological media. Murashige and Skoog vitamins for plant culture come to mind, as one example.
Please share any thoughts or experiences you might have towards identifying substitutes for YE.
Thank you,
Jeff Fuhrmann
Hi Professor Jeffry J. Fuhrmann ,
The purpose of adding yeast extract to culture media is providing vitamins and free amino acids, especially those belonging to the B complex. As it is just like a representation of a multivitamin solution, I hope it could be replaced by currently available multivitamin tablets. I have tried using diluted multivitamin tablets for culturing my rumen bacteria which worked well for me.
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