Jeffry J. Fuhrmann

6 Questions 8 Answers 0 Followers

Questions related from Jeffry J. Fuhrmann

Best methods for collecting/storing bacterial cultures for RNA transcriptomics?

I'm a newbie to RNA work and am seeking advice on methods to sample, process, and store bacterial broth cultures for transcriptomic sequencing. Thanks in advance for your input!

24 June 2025 8,005 2 View

Is anyone aware of defined growth medium for Bradyrhizobium?

I'm trying to track down a defined growth medium for soybean bradyrhizobia (Bradyrhizobium japonicum, B. diazoefficiens, & B. elkanii) but not having much luck. If you are aware of one,...

17 June 2019 5,029 3 View

Isolating large amounts of phages from bacterial cultures free of contaminating DNA

Hi all. I am trying to collect high numbers of intact phages from bacterial cultures while avoiding contaminating DNA from the lysed bacteria; this is for eventual PacBio sequencing of the phage...

16 July 2016 7,288 6 View

Does anyone know the effect of low temperature on DNA gel electrophoresis?

I'm particularly interested as to whether it affects band separation. I've known researchers in the past who sometimes ran gels in a cold room, but I don't know why they did this

22 March 2015 6,923 4 View

Does anyone have a 1980s-era spiral plater manufactured by the Spiral Systems Company?

The model I have is "DU", and the manual for the unit has been lost. I *really* need some information out of the manual, esp the "counting arc-to-volume deposited" translation table. Thank you...

11 July 2014 1,549 0 View

Possible substitutes for yeast extract in bacterial cultures

Hi everyone, In our work with Bradyrhizobium spp., we normally include yeast extract to supply growth factors. However, in epifluorescence examination of cultures, the yeast extract causes high...

01 January 1970 2,482 2 View