Some lanes look like they might have a second band if you ran your gel out for longer. Doesn't look like RNA degradation, as the bands are relatively sharp and the lanes non-smeary.

The concentration is too high. You can dilute it 10 to 100 times.

HI,

The gel is run for less time, so the integrity of the samples/RNA quality cannot be assessed. At very less mobility bands will be apparently sharp, their true assessment will be only with more separation and that too on a low percentage gel. For example sample/lane no 9 seems to have degraded RNA (compared to others). A well run gel image will be more informative, and for quality (integrity) too much dependence  on nanodrop (or similar instrument) is not a good idea.

bye

I suggest reducing the concentration !

Thanks 

you repeated the process again very care fully. I hope you find the better result

Hi 

I experienced such a result when I was trying to extract RNA from trees like Apple, and Pear using Trizol reagent. But I couldn't succeed it unless I used the modified CTAB method.

There are some reasons, leading just one band of RNA that I think high secondary metabolites of some plants are most important factor causes this result.

I agree with most comments here and that is that you need to run your gel longer. Also, you might want to run a a BE gel (Boric acid, EDTA) when running RNA and use a denaturant - I've used methyl mercury (Be careful!). 

run further! even the markers are not separated well enough.

As some of the colleagues already mentioned: do run your gel further to properly evaluate if there is only one band! With RNA i usually run a 1% Agarose Gel as well and do not have any troubles with it, however, when running RNA you could also try running the gel in TBE buffer since it's capacity is a bit higher compared to TAE.

I had the same problem. I think the gel might be too light, I'd recommend 2% agarose gel. Let it run for a longer duration. 1-2 hours. I can see that some bands (2nd and 8th) have not fully separated. I don't think the problem is polyphenols though because if it were so, there would be no bands or it would show as smears. Another thing probably mutations occurred in your samples i.e deletions and insertions and hence what you are getting.