Positive control of apoptosis and autophagy

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The criterion about maximally localized wannier function?

I heard that as the value of "num_iter(tag in wannier 90)" is higher, spread of Wannier function(=WF) is gradually lower in wannier 90. If so, is this procedure that minimize the spread of WF...

13 July 2024 4,608 0 View

Seeking Feedback on Methodology for Developing Dynamic, Cost-Aware Resource Allocation Algorithms in Multi-Cloud Environments?

Hello fellow researchers, I am working on optimizing resource allocation in multi-cloud environments through dynamic, cost-aware algorithms. My approach involves developing real-time adaptive...

25 June 2024 6,006 2 View

Wannier90 error: Could not find projections block in wannier90.nnkp?

Hello ~ I'm currently beginner using Wannier90. My target molecule is "EuTiO3". The electron configuration of this molecule is as follows: Eu: 1s2 2s2 2p6 3s2 3p6 3d10 4s2 4p6 4d10 5s2 5p6...

06 May 2024 2,066 1 View

The relationship between Partial charge & Lowdin charge in Quantum espresso.?

Hello~ I got Lowdin charge distribution from Quantum espresso calculation. But I have a difficulty in converting from Lowdin charge to partial charge... The formula is " (partial charge) =...

24 April 2024 9,487 0 View

How to prevent metal atoms in the active site from forming any bonds with the ligand by using glide in Maestro?

I'm docking an enzyme by using maestro, there is an metal locate at the docking active site, I have already tried a few methods but there are still show the metal is interact with ligands in...

18 April 2024 4,037 4 View

How to improve the nitrogen use efficiency of rice in your contry?

01 April 2024 3,583 3 View

What does it mean meta-analysis P-value, I2 ?

I have had meta-analysis and p-value= 0.8720 I2 =0% used program was Medcalc is it have meaning? and is it associated with p-value and I2 value?

26 March 2024 4,042 4 View

How to cite a paper without the page number information?

In an manuscript, I need to cite a paper. Since the article was recently published and the page number information has not been updated in Web of Science, so I can't get a full quote for it. how...

18 March 2024 4,153 1 View

What are the reasons of instability in the process of laser plasma expansion?

What factors or physical mechanism will affect the laser produced plasma (LPP) expansion process. How to reduce the instability of multiple measurements?

22 February 2024 2,191 3 View

Code for finite element analysis that allows for the specification of some of the mesh node coordinates?

I am looking for a code (open source preferably) for finite element analysis that allows the user to specify some of the node coordinates of the mesh. The code should be able to generate and...

05 February 2024 6,267 3 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

How to represent Annexin V apoptosis study data in publications?

Hi, I have performed an Annexin V apoptosis study using flow cytometry and got quite good results. I doubt how to represent the quadrant percentages in the final bar graphs. Some papers only show...

13 July 2024 356 3 View

Apoptosis and MTT Assay ?

Kindly confirm can we do MTT assay for quantification of apoptosis. Is MTT Assay a valid assay to quantify apoptotic cells ? What about LDH assay ?..how is it better for apoptosis quantification...

08 July 2024 5,482 2 View

THP-1 cells completely dying with PMA, help?

Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...

03 July 2024 3,884 5 View

What could be the expression profile of alkaline phosphatase from lactic acid bacteria?

I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...

02 July 2024 5,332 3 View

Can I do IPTG induction on OD600=2.6 in TB?

I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...

30 June 2024 6,502 5 View

THP-1 differential markers (M0, M1 and M2). what do you recommend?

Hello, what THP-1 differential markers do you use in the Western-blot method? I am looking for proteins that are characteristic of M0, M1 and M2. Do you have something proven and reliable? Greetings

24 June 2024 9,025 0 View