How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

High pass filter on EMG after Bandpass(Boonstra2012)?

Hi all, I am currently trying to carry out an EMG coherence analysis, prior to this I have been reading some papers. I can understand the Bandpass implication from range A Hz to B Hz, but from...

25 November 2020 7,461 1 View

How can I design resin column for test?

I'm interested in the research field of ion exchange resin. I want design about resin column with acryl for test. How do you usually design the diameter and length of small columns for test? I...

13 October 2020 3,443 2 View

How to purify a recombinant protein from Ecoli to reach a cell culture purpose?

05 October 2020 502 7 View

How do I change to make an insoluble recombinant protein to a soluble protein?

How do I change insoluble recombinant proteins to soluble proteins? My recombinant protein is Aldose dehydrogenase from bacterial with around 35.0 kDa size. Now, My expression vector and...

05 October 2020 1,840 2 View

Can XRD determine inorganic material composition?

From my understanding, XRD is used to determine phase identification of a crystalline material. Is it possible to use XRD to determine the composition of a certain material? I think XRF or EDS is...

15 June 2020 9,211 8 View

What are the steps before perform Normality Test?

Hi all, I m a Master Students. I need to perform Normality Test in my study. I have 5 independents variables and 1 dependent variable. Each independents have 6-8 questions and dependent variable...

14 May 2020 9,015 3 View

How to find the model of estimation between 2 borehole?

I am currently writing thesis for my undergraduate where i need to design a machine learning model to estimate the soil data between 2 borehole based on the bore log data. Sorry in advance if i...

09 April 2020 211 3 View

How to fit the Gaussian curve based on the moment of power spectrum?

I want to make a spectral correction curve of my light source to resemble the Gaussian spectrum. This literature said, ".. The dashed curve is a Gaussian fit with a center wavelength of 833 nm...

20 January 2020 8,950 2 View

Polyurethane film stripping problem?

29 December 2019 8,938 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

What is the best concentration of Pam3CSK4 to coat a dish to activate with THP-1?

Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...

21 January 2021 2,593 1 View

FLICA assay not working?

Hi everyone, I am trying to perform the FLICA apoptosis assay with my MSCs. However, although the cells look healthy upon inspection and the viability is greater than 90% with cell counting, the...

17 January 2021 6,959 1 View

Can I use 24 wells black tissue culture uncoated plate for cultuing macrophage (THP-1 treated with PMA) ?or I have to coat them?

Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...

05 January 2021 4,214 3 View

How can I change the media for THP-1 cells without dislodging them from the surface of my well plate?

I am working with THP-1 cells at the moment and am having trouble with maintaining a high confluence once I seed them on a 24-well plate. I usually try to seed around 100,000 cells per well and...

29 December 2020 1,567 3 View

Can anyone tell me if there are any apoptosis resistant cancer cell lines available other than the Bax/Bak dKOs?

I am working with apoptosis independent cancer cell treatment and what I found online included specific drug resistant or apoptotic proteins knock out cell lines. Are there any established cancer...

24 December 2020 3,674 2 View

Is there any chemical or surgical method to induce hypoxia in mouse model?

Dear all, My question is about Inducible hypoxia in vivo (mouse model). I'm looking for some chemicals that can induce the pathway of hypoxia or some other techniques. If you now know something...

15 December 2020 866 6 View

How to avoid non specific bands in western-blots..?

I am working on autophagy.. trying to check the release of free GFP after autophagy by western blots.. but i am getting non specific bands.. how to avoid them and develop clean and neat blots ....?

14 December 2020 7,377 8 View