Polysaccharides that were precipitated with ethanol could not be redissolved with water, why?

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Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

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Diltuions for a Western blot?

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Abdelkader BOUAZIZ

Dear Decha Sermwittayawong, this free downloadable chapter deals with the solubility of polysaccharides. My Regards

https://www.intechopen.com/books/solubility-of-polysaccharides/polysaccharides-structure-and-solubility

Fatma Al-Rubaiai

This is what I also observed during my natural product research. Some compounds might undergo change at their functional groups which in turn affect their solubilities.