Decha Sermwittayawong

6 Questions 7 Answers 0 Followers

Questions related from Decha Sermwittayawong

How can I create insulin-resistant 3T3-L1 adipocytes?

Hi, I am interested in creating insulin-resistant 3T3-L1 adipocytes using TNF-alpha, and I have the following questions: 1. Would it be best to use human TNF-alpha or mouse TNF-alpha? 2. What is...

31 August 2021 6,580 13 View

Could anyone help with a 2-NBDG experiment on C2C12 cells?

I have been working with the C2C12 myotubes and measured their glucose uptake activity using an indirect glucose uptake assay (measuring how much glucose is left in the medium after stimulating...

24 November 2020 2,252 3 View

Polysaccharides that were precipitated with ethanol could not be redissolved with water, why?

Hi, I am working with polysaccharides from plant samples. One of the purification steps is to add ethanol to the water extract to precipitate polysaccharides. However, in some cases, the...

18 March 2020 8,594 3 View

Does anyone perform neutrophil phagocytosis experiment with Candida albicans?

Hello, I am doing an assay on neutrophil phagocytosis using C. albicans. Let say I have two sets of experiments: the untreated and the treated sets. I would like to compare the phagocytosis...

27 July 2015 7,719 3 View

How can I reactivate inactivated DEAE Sepharose Fast Flow?

Hello, I have been using DEAE Sepharose Fast Flow to purify carbohydrates many times and it seems to lose its binding capacity. I washed the resin with30% isopropanol, water, 5% acetic acid,...

10 October 2014 9,906 5 View

Who is familiar with packing a sephacryl S-300 HR column?

I have a problem with my Sephacryl S-300 HR column. I recently purchased the resin to pack with a 70-cm long glass column (homemade), with a 1.75 cm diameter. The bed volume is about 150 ml and...

25 September 2014 2,968 5 View