Hello, I am using Coloc2 in ImageJ to quantifiy the colocalization between two fluorescent channels in my confocal images. The problem is that I should be able to indicate the value of the Point Spread function in my images, in pixels. I don't know what this value is and I can't find it while searching into the properties window of my images.

Is there any Coloc2 user who faced this problem and can suggest me how to solve it? Thanks

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