Phorbol 12-myristate 13-acetate is usually used in cell culture as a tumor inducer. What is a common solvent for PMA if eventually it must be added to a cell culture with medium?
Hello Bill James
PMA can be made in DMSO solvent as a stock solution. Stock solution may be aliquoted and stored at -20°C. You may then prepare working solution to be used in cell culture in serum free medium or aqueous buffer. The final concentration of DMSO in the culture should not exceed 0.1% as this is considered to be safe without any toxicity for almost all cells.
Best.
Dear Bill James,
Look over the following info:
Considerations regarding use of solvents in in vitro cell based assays
Michael Timm, Lasse Saaby, Lise Moesby, and Erik Wind Hansen
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967611/
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Culturing and differentiating THP-1 cells
https://nanopartikel.info/wp-content/uploads/2020/11/cell_culture_THP-1_hic_V1-1.pdf
Dear Bill James . See the following useful RG links: https://www.lyondellbasell.com/globalassets/documents/chemicals-technical-literature/lyondellbasell-chemicals-technicalliterature-glycol-ether-pma-technical-datasheet-2255.pdf
Article Phorbol ester phorbol-12-myristate-13-acetate promotes ancho...
Kindly see also the following useful links: https://www.sciencedirect.com/science/article/pii/S0006497120810179
https://www.invivogen.com/sites/default/files/invivogen/products/files/pma_tds.pdf
Also check please the following useful links: Article Propidium monoazide pretreatment on a 3D-printed microfluidi...
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/816/596/p8139pis.pdf
The following links are also very useful: Article Dimethyl Sulfoxide (DMSO) Decreases Cell Proliferation and T...
https://www.sciencedirect.com/topics/chemistry/phorbol
Visit kindly the following useful RG link : Article Differential effects of phorbol ester (PMA) on blocker-sensi...
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