Plz any one hlep, another crystallisation condition is 40%MPD, 0.2 DIAMMONIUM PHOSPHATE possibility of salt or protein?

Pachineella Lakshmana Rao @Pachineella_Lakshmana_Rao2

12 December 2016 1 7K Report

i am getting big crystals by increasing MPD from 40% to 60%, is the possibility of getting mpd crystals or they are protein crystals, those crystals are not well formed plz give suggestions.

Ziguo Zhang

high concentration of MPD can force many stuff to xtalize. The xtal in your condition is likely to be phosphate salt xtal.

Badges
Science topic

More Pachineella Lakshmana Rao's questions See All

How to build m-RNA secondary structure by using bioinformatics tools ? I need the structure in the pdb format?

RNA structure prediction

01 February 2018 4,000 0 View

Why we have to use Gd.HCl and Urea were used for unfolding studies of protein but not others?

why only these denaturants used why not others can anybody give explanation for this?

01 February 2017 6,630 0 View

How can i calculate free energy between prtein and ligand?

I have done molecular docking of one protein and ligand but i don't know how do i have to calculate free energy in between these two molecules? can you suggest anyone commands related to this?

11 December 2016 2,214 1 View

Gingkotoxin dissolving or preparation?

Please can any one suggest me preparation or how to dissolve gingkotoxin for my experiments?

09 October 2016 4,949 4 View

Protein Dimer modeling?

i have protein dimer sequences of chain A and B, how can i model these two sequences? i want to get as dimer. is there any software ? can you suggest?

09 October 2016 7,125 1 View

Metal incorporation in Autodock?

How do we have to incorporate metal in model structure of protein? is there any option in autodock? i want to do docking small ligand along with metal?

09 October 2016 9,376 1 View

Molecular simulation and dynamics of DNA & Protein?

I have one transcription factor, so how do i have to do dock with DNA? how can i create PDB file of DNA? what is the procedure for simulation in gromacs? can you suggest anyone?

09 October 2016 1,172 0 View

Protein-ligand simulation?

i have modelled one protein by using modeller, after docking protein with suitable ligand, it is interacting with five amino acid residues, then i submitted that complex to 20 ns simulation...

08 September 2016 9,778 3 View

Molecular dynamics and simulation?

Basically how many nanoseconds we have to use for protein simulation? i used 20 ns for modeled structure, is it sufficient or shall i increase the time? can you suggest anyone?

08 September 2016 5,630 8 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

I have get this error when i calculated the geometrical optimization for prco3, it takes 12 hours until gives this message in the outputfile?

Fatal error in MPI_Allreduce: Other MPI error, error stack: MPI_Allreduce(1628)......: MPI_Allreduce(sbuf=000002459254A180, rbuf=000002459F86A140, count=4851, MPI_DOUBLE_COMPLEX, MPI_SUM,...

09 August 2024 7,615 1 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

What is the prepared reference material that can be used in inductively coupled plasma optical emission spectrometry (ICP-OES) to measure REE and U ?

What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...

06 August 2024 1,896 1 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal?

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal? Kindly advice

04 August 2024 7,367 3 View

Are authors sowing for scientific Journals to be reaping the benefits? We are charged for publication but we offer free peer review services, why?

As authors and academic writers, we usually prepare our manuscript using our own resources. We submit these manuscripts to scientific Journals for peer review and publication sometimes at a fee....

03 August 2024 7,304 2 View