Access to certified lab to confirm the presence of forever chemicals in Nigeria has been difficult. Hence, there is limited research in this area.
My transduced cell can resist Puromycin and 99% express GFP, but it cannot show me the difference in the knockdown. What could be the possible reason?
31 May 2024 454 2 View
what are the possible reason(s) that make AsPC-1, 293T and MCF-7 cell lines failed to be transfect/transduced?. Please help me with possible hints?
10 March 2024 8,812 2 View
Please i have been trying to dissolve Palmitic acid in BSA-NaOH, it keeps solidifying after heating. I wanted to used DMSO but its' lethal to cells. And also the DMSO recommended concentration to...
25 September 2023 990 1 View
We're performing genetic diversity studies using SSR and RAPD markers and we're done with the experiments, but we're finding it difficult to get this software for the data analysis.
06 May 2023 6,728 3 View
I would greatly appreciate someone assisting me with guiding documents for broth microdilution and agar dilution. Thank you.
24 November 2022 362 1 View
For a synthesized complex compound, why do determine its maximum absorption using spectrophotometer?
17 February 2021 7,544 3 View
So many journals claim they are indexed in WoS (ISI in old parlance; now called Clarivate Analytics). However, most of the journal end up listed in the Zoological Record (ZR). Reading about that...
03 March 2020 2,040 3 View
I am working on detecting the presence, quantity of mycotoxins as well as the fungi associated with it from dried tomatoes.
24 December 2018 3,177 5 View
Please somebody should help me with idea on how to prepare 0.1molar solution of sodium hypochlorite from its stock with 14-15% chlorine for kinectics studies
21 November 2018 2,753 0 View
I want to measure the Level of IgG, IgM, IgA, and IgE in rabbit model. I mistakenly lost my serum samples but i have some blood samples saved in K3-EDTA tubes. So i was thinking that i can...
22 May 2018 3,696 1 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...
08 August 2024 6,690 1 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...
05 August 2024 5,829 1 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Why is nonpoint source pollution potentially more harmful and difference between point and nonpoint sources of water pollution?
01 August 2024 1,180 2 View
I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...
01 August 2024 8,368 2 View