My explants keep getting contaminated even with all the protocols used
I will be grateful if i can get the latest work on the above topic.
17 May 2024 2,509 1 View
My temperature is at 20 degrees ees and my humidity at a range of 45 - 50 my plants are not growing
28 February 2024 4,398 1 View
some yellow, orange and white microbes were seen on some of the media. I'm not too sure about the type of microbe whether bacteria or fungi. I would like to know whether I can add antibiotics to...
29 November 2023 3,511 1 View
dd2 file or vdf file.If so how to find the absorbance value.Thankyou in advance.
23 January 2022 3,784 2 View
I am using data of 25-year interval, one dependent variable and 5 independent variables, all are time series in nature. I would be grateful for a step by step help in the process of analysis with...
12 February 2019 6,734 4 View
for instance the LOD for Aspartic acid is 2.33 pmol/µL, the moleuclar wt is 133.10 g/mole, and LOQ is 7.07 pmol/µL, the protein was hydrolysed in 50 mL solvent and from which 1 µL is injected to...
05 December 2017 2,355 3 View
We were trying to identify reducing sugars from one of the African tubers. We used GC varian and it went well, identifying fructose, glucose, sucrose, and maltose. However, the problem is that we...
01 June 2014 4,428 21 View
I mean the approximate timing for those surface?Is there another way to generate the hirshfield surface
01 January 1970 8,679 1 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...
11 July 2024 7,786 3 View
There are black dots that seem like contamination in my corneal endothelial cell cultures. Some of the cells appear unhealthy and are enlarged. The black dots are more prevalent around these...
11 July 2024 4,829 0 View
The question refer to leafy biomass decomposition using litterbag techniques.
11 July 2024 2,078 0 View
When Streptomyces undergoes conjugation transfer, it is always contaminated by a type of yellow Pseudomonas. What could be the reason for this?
07 July 2024 5,480 2 View
What is meant by cross-contamination and what causes fruits and vegetables to become contaminated during preparation and storage?
02 July 2024 9,204 0 View
We have been experiencing repeated what looks very much like fungal contaminations in several incubators (in the same lab). We have tried the following repeated times and yet the coating on all...
02 July 2024 6,375 2 View
Within our research lab we have access to PFOA in powder form and PFOS in 100microgram/L methanol solution. We want to make a PFAS contaminated water to test a PFAS treatment technology. Does...
01 July 2024 8,425 3 View
