some yellow, orange and white microbes were seen on some of the media. I'm not too sure about the type of microbe whether bacteria or fungi. I would like to know whether I can add antibiotics to repress their growth and ways to apply it
The fact you are getting this contamination means there needs to be an improvement in lab hygiene and/or sterile technique used to make the media. Have you sterilized all surfaces used for media preparation? How are you pasteurizing your media (some details on the media preparation protocol would be useful)? In general you put the cooling media in a water bath at 50 degrees centigrade and then add the antibiotics just before pouring the plates. To know which antibiotics will work against your contaminants will be trial and error. You must also be careful that they do not inhibit your teak teak culture. Nipagen is a good broad spectrum antibiotic.
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