Is there no need to labell the dye with the primer ?
Master Mix given by Edgardo E Salvatierra
Mix Conc. ul
h2o 15.15
Buffer 10 X 2.5
MgCl 50 mM 1.5
dNTPs 10 mM 0.5
Primers 5 uM 2
BSA 10 mg/ml 0.25
Taq plat 5 U/ul 0.2
Sybr (10x) 10 X 0.4
Rox (5x) 50 X 0.5
Total: 23
cDNA 2