I want to synthesize the silica gel layer used in spin columns.
In metal oxides how oxygen affect the electrical and magnetic properties? Also, how it impacts the EMI shielding performance. Please make discussion.
05 June 2024 483 0 View
I want to make reconfigurable RF and microwave filter, I need to connect low capacitance (minmum-0.048pF) varactor diode (MA46H120 Series). Can we use this varactor diode (MA46H120 Series) at 6...
21 May 2024 7,504 2 View
We have elastic scattering excitation function data in tabular form and want to obtain partial wave scattering phase shift data for each partial wave say l=0, 1, 2....whats the process to do so...
19 April 2024 2,225 1 View
I have used the DLS machine (Zetasizer Software) to measure the size of micelle prepared. For the first 2 runs the quality report shows "Good", while the 3rd measurement shows as "Too...
17 April 2024 4,491 3 View
I am working on identification of copy number in a recombinant Chinese hamster cell line by southern hybridization. I am having issues with the transfer of DNA onto positively charged nylon...
08 February 2024 2,647 8 View
We did reaction of alpha-bromo-gamma-butyrolactone with aq. ammonia solution at RT to get alpha-amino-gamma-butyrolactone. Reaction completed on TLC. Water distilled completely but unable to...
07 February 2024 5,766 0 View
I tried different protein assay kits (BSA, fluorometric peptide), but it didn't work.
25 January 2024 3,341 0 View
For example, Data obtained from a rheometer is as follows 1. Static yield stress - 1400 Pa, 2. Dynamic yield stress- 640 Pa, 3. Plastic viscosity-68 Pa-Sec, How are these values exactly...
24 January 2024 6,120 0 View
while working with low concentrated mammalian genomic DNA, can we increase the restriction digestion reaction volume to 200 ul to accommodate the required amount of mammalian genomic DNA for...
22 January 2024 6,952 12 View
Dear Researcher Happy New Year-2024. The world is facing different types of health issues today, among all Malaria is one of them. from hundreds of years scientist are working for the controller...
31 December 2023 1,619 3 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
