Dear Sir. Concerning your issue about the best method to separate buparvaquone in tissue by HPLC. A high performance liquid chromatographic (HPLC) method for the determination of the antitheilerial drugs parvaquone and buparvaquone in plasma was developed. Both compounds were extracted from plasma with ether. After evaporating the extracts to dryness the residue was dissolved in methanol and an aliquot was injected onto a column (10 cm x 5 mm, i.d.) of ODS-Hypersil (5 u) with a mobile phase of 0.05 M - Na acetate buffer (pH 3.6) - methanol (15:85. v/v). Detection was at 252 nm. The mean recovery for both compounds was about 92%. This method was used to elucidate their pharma¬ cokinetics in 6 calves after intramuscular administration. The maximum
plasma concentration for parvaquone was 6.36 ±0.58 |ag/ml after 0.84 ±0.08 h. The corresponding values for buparvaquone were 0.102 ± 0.030 ug/ml and 3.17± 0.39 h, respectively. The decay in plasma concentrations for the two drugs was biexponential and the terminal elimination half lives were 11.12 ± 1.63 h and 26.44 ±2.81 h for parvaquone and buparvaquone, respectively. I think the following below link and the attached file may help you in your analysis: