Hi Linda,

in order to quantify intensity you could try both imageJ or may better open source software CellProfiler, it's not intuitive but few days of practise and it will work.

Good luck

Hi, to confirm Cesare you can use the image J software

It's simple, easy to work and it will help you to determine the color intensity.

good luck

@ cesrare : please can you write me in detail ho can i use it ??

Thx in advance 

Hi Linda,

for CellProfiler you need to set up your ipelne according to your images and in particular to mineralized nodue size.

Rgarding imageJ open your images go to anazlyze set measurements select the variable you need to score, then select the areas to score and back to analyze and click to measure.

Good luck

Hi!

We extract the coloration with a solution of cetilpyrimidium and then read it at 562nm. You will have to normalize with DNA amount in each well.

GL

Hii

Please find attached herewith a publication that gives quantitation of mineralization using the cetylpyridinium chloride method.

All the best!!!