Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

Bacterial Contamination in Laminar Flow Hood?

Hi there! Brace yourself, this is going to be long winded and contains a year's worth of frustrations. I culture moss, and for the last year, I have been fighting a gram negative bacterial...

29 August 2020 6,492 2 View

Has anyone used Python for qualitative research analysis?

I am looking into the benefits of using programming skills for data coding and analysis. This stems from the need for highly technical skills when considering roles outside academia. If you have...

23 June 2020 9,142 1 View

Pittsburgh Sleep Quality Index Scoring Synthax on Excel?

Hi, Just checking if anyone has an Excel scoring synthax for the Pittsburgh Sleep Quality Index that they would be happy to share Thanks Kate

24 March 2020 4,047 2 View

Can I Combine Data and Tree Information into One Tree in Mesquite?

Is it possible to enter two different types of information into Mesquite? I have two sets of species data: the divergence times (in millions of years) i.e. how old each species is and the...

22 March 2020 6,304 3 View

Can phenomenography be used even with limited variation in the data collected?

I am currently conducting a study and have collected interview data from students on their perspectives and experiences. I had anticipated that these experiences would be different based on their...

06 January 2020 9,667 2 View

What would be an appropriate parent-report tool for measuring circumscribed interests in young children with autism?

I'm looking to get an idea of the intensity of circumscribed interests for young children (3-6 years) with autism. Does anyone know of an appropriate parent questionnaire that can be used to...

14 January 2019 7,013 3 View

Any tips for faster EEG setup with ActiCap - particularly with kids?

I am running a study at schools and need to try to fit as many kids in a school day as possible. The EEG setup is taking a while; I already have them wash their hair with shampoo only that...

11 August 2018 1,041 1 View

Are the random primers only for first-strand cDNA synthesis or can you do one PCR too?

I already did my RT, but now I want to do a PCR to replicate all my cDNA, is it possible to do a PCR with the hexamer random Primer as well? I do not find any KIT for PCR that is not with gen...

13 June 2018 6,254 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

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01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View