Could anyone help to find the method for stomach ulcer induction in rats according to Shay et al. or published work on it more in detail?
Hi Nodriali,
I found one protocol for establishing acetic acid induced chronic ulcer model in rat. The attached papers are good resources for further reading which I believe will help you developing the protocol.
Best-
The best ulcer model for the rat is as follows:
Okabe S, Amagase K / An overview of acetic acid ulcer models. The history and state of the art of peptic ulcer re-search // Biol. Pharm. Bull. 2005;28(8):1321-1341
If you are interested in stress, one very good method is to restrain them at room temperature or in the cold.
Dear collagues, thank you very much for your support. It seems the article offered by Andrey Borodach is very informative for us. Thank you again.
Dr.Normakhamatov,
I dare to recommend my own review article on this matter here
https://www.researchgate.net/publication/258821777_Ulcer_disease_the_state_of_art as well. I would be highly obliged if you post your opinion about it.
Good luck!
Article Ulcer disease: the state of art
Ulceration in the rats was induced with a single dose of indomethacin (30 mg/kg BW., oral gavage) dissolved in DW. The rats were deprived of food but had free access to tap water 24 h before ulcer induction
the ulcer in rat is induced by various methods , these are
ethanol induced ulcer, acetic acid induced ulcer , stress induced ulcer, pylorus ligation induced ulcer, indomethacin induced ulcer, aspirin induced ulcer, naproxen induced ulcer. The models are vary according to the severity like acute, chronic or subacute. You can use acetic acid induced ulcer model, with that you can give long treatment to rat by giving the indomethacin daily for 20 days it will produce chronic unhealed ulcer. also you can infect that ulcer with H-pylori to mimic the ulcer condition which usually occur in human being after H-pylori infection.
Another great method is cysteamine administration. Please look at publications by S. Szabo et al.
Dear Milagros,
Thank you for sharing your knowledge. Really, the cysteamine method seems very simple and effective.
Also inducing gastric antral ulcers by the administration of 1.0 M HCl after refeeding for 1 h in rats.
also gastric ulceration was induced by a single intragastric administration of indomethacin (18 mg/kg)
Dr.Salas-Prato,
Would you share the complete reference to the article by Szabo you mentioned above here, please?
Dear Dr. Andrey Borodach, I hope you can download this article from here. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2018340/
I have read the article and would like both to say thanks again for the colleague uploaded it and to ask a couple of more questions to advanced researchers of the experimental peptic ulcer disease:
1) The author did not explain why the ulcer developing after the cysteamine hydrochloride treatment displayed itself as a quite local defect with sharp borders, not as multiple or irregular ulceration. What is your opinion?
2) Judging by available literature, the cysteamine does not produce gastroduodenal ulcerations in humans, does it not?
I shall ask Sandor for the specific articles or look them up in Pub Med. Also, you can please look up Dr. Szabó here in Res. Gate.
Dr.Salas-Prato,
It is pleasure to read the answer of yours.
Yes, I would be gladly ask my questions to Dr.Szabó if this is appropriate.
Thank you. Likewise. Yes, of course, go ahead, I already asked him too.
Here is one of his latest reprints on this which should also cite all of his former work.
Also, as I mentioned, if you are interested in the restraint-induced ulcer model, please see my stress publications in Res. Gate.
New mechanistic explanation for the localization of ulcers in the rat duodenum: role of iron and selective uptake of cysteamine. Khomenko T, Kolodney J, Pinto JT, McLaren GD, Deng X, Chen L, Tolstanova G, Paunovic B, Krasnikov BF, Hoa N, Cooper AJ, Szabo S.
Arch Biochem Biophys. 2012 Sep 1;525(1):60-70. doi: 10.1016/j.abb.2012.05.013. Epub 2012 Jun 7.
PMID: 22684023 [PubMed - indexed for MEDLINE]
The methods added is included file mention how to induced peptic or gastric ulcer
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