Dear Tejas

I think if you can buy and use CLCBIO software you can do all of assemble sequences with it.

I also tried it..........thats ok but can u suggest parameter for assembly............ Most of publication of plant transcriptome study used hydrid contigs or super contigs............. So wt is the criteria for identify good quality contigs????

if you contact to Dr Ebrahimie he can help you. his email is: [email protected]

Thank you very much....... I will contact him........