Yesterday, I prepared the minimum medium Yesterday, I prepared the minimum liquid medium for the screening of bacteria that have the ability to degrade naphthol and use it as a single carbon source which is composed of:
1,0 g K2HPO4-2H2O
1,0 g KH2PO4
5 g NaCl
0,3 g (NH4)2SO4
0,3 g MgSO4-7H2O
20 mg CaCl2
5,0 g ZnSO4
2,3 g FeCl3
5,0 g MnSO4
1,0 g (NH4)6Mo7O24
This is the composition that suits my bacteria, I was inspired by this article: https://sci-hub.se/10.1016/j.jhazmat.2010.06.101
Biodegradation of naphthalene by strain Bacillus fusiformis (BFN)
but the problem is that after autoclaving, there was the precipitation of the components at the bottom of the bottle .
can I add before autoclaving a solution of cacl2 so that there is no precipitation? and how much can I add of this solution in 1 liter of minimum media ?
Please give me all the tips I can do or avoid to succeed in this medium
Than you .
Dear Manel Moumene,
Try to prepare the medium by fractioning the solutions, one way in this particular case would be to prepare four solutions:
1. Phosphate salts (adjust the pH of this solution),
2. NaCl, Mg, Ca and sulfate ammonium,
3. Zn, Fe, Mn and Mo
4. carbon source solution.
Sterilize separately and collect them all at room temperature. Storing the reconstituted medium in the refrigerator can generate precipitation, so I do not recommend preparing large volumes.
Good luck
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