How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Is there such a thing as Igg specific MHC class II epitopes or do all non-self MHC class II epitopes trigger antibody production of all subtypes?

I know there are bioinformatic tools that allow epitope prediction to determine if a certain peptide would trigger an immune response. I also read that for allergen diagnoses IgE of sensitised...

09 February 2021 4,173 3 View

What if design and develop some mechanism to eradicate the triggers to achieve data accuracy and achieve assured results?

The realistic approach to identify the exact choices and or preferences is quite difficult seems due to multiple unrealistic profiteering triggers applied to confuse and deviate the end-users from...

25 December 2020 8,304 1 View

What's the difference between Photoacoustic (PA) and Photoacoustic Flow Cytometry (PAFC)?

I was asked to do a presentation on the following paper: Juratli, Mazen & Menyaev, Yulian & Sarimollaoglu, Mustafa & Siegel, Eric & Nedosekin, Dmitry & Suen, James &...

14 October 2020 8,346 1 View

What would be the likelihood of different environmental trigger for the same virulence operon across isolates?

So, I am looking at condition regulating the expression of a group of virulence gene. I have several genes, all supposedly on the same operon, and, sure enough, they all seem to react together...

25 August 2020 1,753 3 View

How to define data in MPlus?

Dear Researchers, I want to validate a scale containing 20 items. I expect to extract four factors. I perform commands in Mplus ( Mplus VERSION 8.4 DEMO) as in the following: Title: EFA in...

14 August 2020 9,375 3 View

What condition causes the binary mixture of triglycerides to produce molecular compound?

The molecular compound was formed in several pairs of binary mixtures of triglycerides (TAGs). Some cases are such: SOS/SSO, SOS/OSO, POP/OPO, and POP/PPO. It was found in ratio 50/50 in all...

26 March 2020 3,535 2 View

Why is R studio constantly running demo data?

Hello I downloaded the TITAN2 package. I'm adding the library for this package with the library("TITAN2"). I have two data files in this package called env.csv and taxa.csv. But when you enter the...

13 March 2020 7,338 2 View

Why traditional (protein/antigen based) vaccines don't trigger a cell mediated immune response ?

Companies like Moderna are working on mRNA based vaccine as they can trigger both cell mediated as well humoral arm of immunity. But why traditional protein based vaccines can't trigger sufficient...

02 March 2020 4,132 3 View

Why traditional (protein/antigen based) vaccines don't trigger a cell mediated immune response ?

Companies like Moderna are working on mRNA based vaccine as they can trigger both cell mediated as well humoral arm of immunity. But why traditional protein based vaccines can't trigger sufficient...

29 February 2020 9,023 3 View

What neurons to be fired to identify positive emotions in an neural network?

we are trying to build a cnn model to classify emotions from music. we know that only certain neurons will be triggered while identifying positive and negative emotions. But we can not identify...

01 February 2020 5,708 3 View