Please can anyone recommend a supplier for Cry and Cyt (from Bacillus thuringiensis)?

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Can we dilute LysoTracker™ Deep Red (pH sensitive probe dispensed in 1mM solution with DMSO) with citrate buffer ?

I need to dilute the already existing 1mM solution but i have to use it with citrate buffer, can any complications occur if DMSO solution is diluted with citrate buffer?

24 September 2023 1,030 2 View

Hello, Why could be the reason for my bands to appear like this?

I am trying to genotype wild type/mutant mice. I used tail snips and 4 different primers

24 April 2022 1,219 2 View

How can I interpret Wilcoxon Rank Sum and Signed Rank Test for Clustered Data?

I have a continuous variable in my dataset and it is not normally distributed. I wanted to check if the continuous variable is different from zero. The observations are not independent. There are...

18 October 2021 4,397 31 View

Regarding control strategy of inverter in solar grid tied inverter ?

I am working on 5kW grid tied inverter. I have performed detailed simulation of the system in matlab simulink. Simulation was done for the case of increase in active power i.e. increase in...

28 January 2020 790 1 View

How to increase antibody penetration in 3D structures formed in collagen gels?

I have intricate mouse mammary tissue organoids that have grown in 3mg/ml rat tail collagen. I would like to do immunofluorescence staining with some specific antibody. I have tried incubating...

04 August 2019 9,509 3 View

Is Nickel a biocompatible material?

As iron is FDA proved material which can be studied for biocompatible test. In that case can nickel be a biocompatible material?

30 July 2018 1,847 3 View

How do you save data generated from PD Quest software?

I am having an issue with storing data generated using Bio-rad's PD Quest software. Once I am done with 2D electrophoresis analysis, I am unable to export my "Experiment Summary Report"....

15 May 2018 9,233 1 View

Please let me know where do I get a bore/tubewell litholog for Saharanpur, Uttar Pradesh?

Required for information on vertical soil profile and aquifer of the region. I could not find CGWB Ground Water Report for Saharanpur district.

24 March 2018 4,265 4 View

Are there any algorithms similar to random forest algorithm?

Are there any algorithms similar or better than random forest algorithm for prediction and classification

21 February 2016 9,209 6 View

How is channel estimation performed in cognitive radios?

Is it in any way related to spectrum sensing, e.g. is it a part of the spectrum sensing process? And what will be the course of action if the channel quality of the sensed spectrum (white space)...

31 December 2013 9,057 5 View

Does it work as antibiotic resistance if plamid has no RBS??

I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...

11 July 2024 7,847 1 View

Is DNase I (RNase-Free) necessary to be used before cDNA synthesis?

I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...

27 June 2024 2,951 5 View

Can someone help me get this article?

..?

15 June 2024 6,072 2 View

How to start purification of enzyme from bacillus licheniformis as I am not getting clear supernatent after centrifugation it feels like jelly in it?

This jelly gets precipitated during ammonium sulfate precipitation

06 June 2024 5,631 4 View

Can anyone offer advice on how to show the inhibition zone of bacillus against s.aureus?

Hi, i am doing research on the potential antimicrobial activity of Bacillus against s.aureus.i have tried various methods for six months.. like soft agar overlay method but my bacillus colony keep...

26 May 2024 2,151 6 View

How to prepare a perfusion buffer and calculate the concentrations of each enzyme?

I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...

02 May 2024 4,928 0 View

Which agars should I use for the microbiological analysis of purified water ?

As a new microbiologist, I am currently working in a non-sterile production pharmaceutical factory where I take pure water samples from 13 points, fill 100 ml of pure water into 3 sample...

29 April 2024 7,096 1 View

Does anyone have a PDF file with the API Catalog (API systems from Biomerrieux) that are willing to share?

I have some environmental isolates that we are looking to identify. Morphologically, the majority of the isolates are Bacillus spp. We would like to use the API 50 CHB/E system and would like to...

28 April 2024 9,559 0 View

Which growing medium is best for the Bacillus Globigii sporulation with maximum yield?

Please provide an answer along with the reference.

26 March 2024 7,488 2 View

What could be the reasons of protein not expressing in Bacillus Subtilis and Bt??

I and using vectors with autoinducibe promoters(Pylb and cyt1). I have performed SDS of 24, 48, 72, 96 and 120 hours. No expression is found in soluble or insoluble form.

14 March 2024 8,752 0 View